ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (6): 1026-1036.doi: 10.11843/j.issn.0366-6964.2015.06.020

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Preparation，Efficacy and Safety Evaluation of Compound Nanoemulsion of Cefpodoxime Proxetil

LIU Yue，LI Meng-yun，ZHENG Yin，GAO Xian，OUYANG Wu-qing*，YANG Ming-qi*   

  1. （College of Veterinary Medicine，Northwest A&F University，Yangling 712100，China）
  • Received:2014-09-09 Online:2015-06-23 Published:2015-06-23

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Abstract:

To study the efficacy of combined application of cefpodoxime proxetil and Origanum oil in nanoscale level，compound nanoemulsion of cefpodoxime proxetil was innovatively prepared.Furthermore，antibacterial activity tests in vitro and acute toxicity tests were conducted.Preliminary prescription was sieved on the base of traditional method of preparation of nanoemulsion.Then the multi-index orthogonal experiment was proceeded with L9（34） orthogonal test table，regarding bacteriostatic circle diameter and nanoemulsion stability constant Ke as examining index.Three elements，ratio of the mass fraction of cefpodoxime proxetil and Origanum oil，pH of aqueous phase and emulsifying temperature are selected as factors.Antibacterial activity tests in vitro and acute toxicity tests were done through trace broth dilution method and maximal tolerance dose test.Then histopathological examination was measured.The optimal prescription is composed of 0.33% of cefpodoxime proxetil，2.67% of Origanum oil，1.13% of 1，2-propylene glycol，22.51% of EL-40，73.36% of distilled water for enteropathogenic E.coli and Salmonella and pH value of nanoemulsion is 6.17.Compared with the active pharmaceutical ingredients，both of the minimum inhibitory concentrations and minimum bactericidal concentrations of test drug were significantly different（P<0.05）；the maximum tolerated dose of the oral acute toxicity of mice is higher than 1 800 mg•kg-1，showing low toxicity.Histopathological examinations show no toxicity of kidney and small intestine，but can do pathological damage to the liver.When in the presence of Origanum oil，enteropathogenic E.coli，Salmonella，Proteus mirabilis，Pasteurella，Shigella dysenteriae，Staphylococcus，Streptococcus agalactiae are more sensitive to CFP-OR-NE than cefpodoxime proxetil.

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